More vector infographics about PNIPAM

A collection of resizable, editable information graphics I used in my Ph.D thesis to explain the various uses of poly(N-isopropylacrylamide) (PNIPAM) for biology and healthcare.

I published earlier some infographics I created for my Ph.D thesis, for example the comparison between biological and technological objects at the micro and nano scale. Below are a few more specific to PNIPAM; they're now also available to illustrate Wikipedia articles about PNIPAM.

Conformational switch (pictured above)

PNIPAM is a thermosensitive polymer that undergoes a reversible coil-to-globule conformational transition around its lower critical solution temperature (LCST), around 32°C. It switches from a hydrophilic, swollen state to a hydrophobic, collapsed state.

PNIPAM-based immunoassay

The peculiar properties of PNIPAM make it possible to use this polymer as a support for immunoassays based on thermal precipitation.

Immunoassay based on the thermal precipitation of PNIPAM

Immunoassay based on the thermal precipitation of PNIPAM

Cell culture

PNIPAM-grafted surfaces can be used as a soft support for cell cultures. Cells grow on hydrophobic PNIPAM and are softly released by lowering the temperature and making the PNIPAM hydrophilic.

    Cell culture on PNIPAM-grafted surfaces

Cell culture on PNIPAM-grafted surfaces

Adsorption and release of proteins

The same way cells adsorb on hydrophobic PNIPAM surfaces, proteins may be reversibly trapped on PNIPAM surfaces, then released upon command. This is actually one of the things I did during my Ph.D.

Controlled adsorption and release of proteins on hydrophobic PNIPAM-grafted surfaces

Controlled adsorption and release of proteins on hydrophobic PNIPAM-grafted surfaces